:Sequel™ NE reagent (Part A)

Sequel™ NE reagent (Part A)

concentrate for sequel™ DNA sequencing solution


20% acrylamide/bisacrylamide (19:1)
8m urea
0.1m tris
0.08m borate
1mm edta
0.075% temed
Unit Min Max
Polymerization Test (Sop-0088) Pass 0 0
Visual Inspection Pass 0 0

Notes & Applications

store at 2°C to 8°C
add ammonium persulfate to initiate polymerization, then cast gel
make any percentage sequencing gel (up to 20%).
Part A contains 20% acrylamide and is mixed with Sequel NE part B to
Sequel™ NE part A is one component of a two part sequencing solution.
for DNA sequence gel
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