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Cod Uracil-DNA-glycosylase
Specifications
Concentration: ≥1u/µl |
Unit | Min | Max | |
---|---|---|---|
Endonuclease (None Detected) | Pass | 0 | 0 |
Exonuclease (None Detected) | Pass | 0 | 0 |
Volume Activity | UNITS/UL | 1 | 1.2 |
Protein (Bio-Rad/bradford) | µg/mL | 0 | 0 |
Specific Activity | UNITS/mg | 1000000 | 1200000 |
Total Activity | UNITS | 100 | 120 |
Notes & Applications
Cod UNG hydrolyses the N-glycosylic bond between the deoxyribose sugar and the base in uracil
containing DNA leaving an abasic (apyrimidinic) site in DNA. Cod UNG is not active on uracil in RNA.
The high activity combined with a fast irreversibly heat-inactivation step makes the enzyme ideal
for contamination control in PCR- and RT-PCR reactions.